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pmlc  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc pmlc
    ( A,B ) Representative immunofluorescence images <t>of</t> <t>paxillin</t> and F-actin (phalloidin) ( A ), and <t>pMLC</t> and F-actin (phalloidin) ( B ) immunostains are shown in inverted black and white (ibw) contrast as well as color composites (DAPI signal also included). Scale bar = 10 µm. ( C ) Compared to WT cells (n=243), lamella size is significantly increased in DLC1 KD cells (n=290, p-adjusted<0.001) and DLC1 KO cells (n=242, p-adjusted<0.001), while DLC1 rescue cells (n=57) show significantly lower lamella sizes than both WT (p-adjusted=0.0098) and KO cells (p-adjusted<0.001). ( D ) DLC1 KD cells (n=290) display a significant increase in the total area of focal adhesions per cell (p-adjusted<0.001) compared to WT cells (n=243), while DLC1 KO cells (n=242) do not show a significant difference (p-adjusted=0.09). Rescue cells show a significant decrease in the total area of focal adhesions (p-adjusted<0.001). ANOVA plus Tukey’s honestly significant difference test.
    Pmlc, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pmlc/pmc13068432-322-16-17?v=Cell+Signaling+Technology+Inc
    Average 86 stars, based on 1 article reviews
    pmlc - by Bioz Stars, 2026-07
    86/100 stars

    Images

    1) Product Images from "GTPase-activating protein DLC1 spatio-temporally regulates Rho signaling"

    Article Title: GTPase-activating protein DLC1 spatio-temporally regulates Rho signaling

    Journal: eLife

    doi: 10.7554/eLife.90305

    ( A,B ) Representative immunofluorescence images of paxillin and F-actin (phalloidin) ( A ), and pMLC and F-actin (phalloidin) ( B ) immunostains are shown in inverted black and white (ibw) contrast as well as color composites (DAPI signal also included). Scale bar = 10 µm. ( C ) Compared to WT cells (n=243), lamella size is significantly increased in DLC1 KD cells (n=290, p-adjusted<0.001) and DLC1 KO cells (n=242, p-adjusted<0.001), while DLC1 rescue cells (n=57) show significantly lower lamella sizes than both WT (p-adjusted=0.0098) and KO cells (p-adjusted<0.001). ( D ) DLC1 KD cells (n=290) display a significant increase in the total area of focal adhesions per cell (p-adjusted<0.001) compared to WT cells (n=243), while DLC1 KO cells (n=242) do not show a significant difference (p-adjusted=0.09). Rescue cells show a significant decrease in the total area of focal adhesions (p-adjusted<0.001). ANOVA plus Tukey’s honestly significant difference test.
    Figure Legend Snippet: ( A,B ) Representative immunofluorescence images of paxillin and F-actin (phalloidin) ( A ), and pMLC and F-actin (phalloidin) ( B ) immunostains are shown in inverted black and white (ibw) contrast as well as color composites (DAPI signal also included). Scale bar = 10 µm. ( C ) Compared to WT cells (n=243), lamella size is significantly increased in DLC1 KD cells (n=290, p-adjusted<0.001) and DLC1 KO cells (n=242, p-adjusted<0.001), while DLC1 rescue cells (n=57) show significantly lower lamella sizes than both WT (p-adjusted=0.0098) and KO cells (p-adjusted<0.001). ( D ) DLC1 KD cells (n=290) display a significant increase in the total area of focal adhesions per cell (p-adjusted<0.001) compared to WT cells (n=243), while DLC1 KO cells (n=242) do not show a significant difference (p-adjusted=0.09). Rescue cells show a significant decrease in the total area of focal adhesions (p-adjusted<0.001). ANOVA plus Tukey’s honestly significant difference test.

    Techniques Used: Immunofluorescence



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    Image Search Results


    ( A,B ) Representative immunofluorescence images of paxillin and F-actin (phalloidin) ( A ), and pMLC and F-actin (phalloidin) ( B ) immunostains are shown in inverted black and white (ibw) contrast as well as color composites (DAPI signal also included). Scale bar = 10 µm. ( C ) Compared to WT cells (n=243), lamella size is significantly increased in DLC1 KD cells (n=290, p-adjusted<0.001) and DLC1 KO cells (n=242, p-adjusted<0.001), while DLC1 rescue cells (n=57) show significantly lower lamella sizes than both WT (p-adjusted=0.0098) and KO cells (p-adjusted<0.001). ( D ) DLC1 KD cells (n=290) display a significant increase in the total area of focal adhesions per cell (p-adjusted<0.001) compared to WT cells (n=243), while DLC1 KO cells (n=242) do not show a significant difference (p-adjusted=0.09). Rescue cells show a significant decrease in the total area of focal adhesions (p-adjusted<0.001). ANOVA plus Tukey’s honestly significant difference test.

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    Article Title: GTPase-activating protein DLC1 spatio-temporally regulates Rho signaling

    doi: 10.7554/eLife.90305

    Figure Lengend Snippet: ( A,B ) Representative immunofluorescence images of paxillin and F-actin (phalloidin) ( A ), and pMLC and F-actin (phalloidin) ( B ) immunostains are shown in inverted black and white (ibw) contrast as well as color composites (DAPI signal also included). Scale bar = 10 µm. ( C ) Compared to WT cells (n=243), lamella size is significantly increased in DLC1 KD cells (n=290, p-adjusted<0.001) and DLC1 KO cells (n=242, p-adjusted<0.001), while DLC1 rescue cells (n=57) show significantly lower lamella sizes than both WT (p-adjusted=0.0098) and KO cells (p-adjusted<0.001). ( D ) DLC1 KD cells (n=290) display a significant increase in the total area of focal adhesions per cell (p-adjusted<0.001) compared to WT cells (n=243), while DLC1 KO cells (n=242) do not show a significant difference (p-adjusted=0.09). Rescue cells show a significant decrease in the total area of focal adhesions (p-adjusted<0.001). ANOVA plus Tukey’s honestly significant difference test.

    Article Snippet: Cells were incubated overnight with the primary antibody 1:250 dilution for paxillin (Abcam ab32084), 1:50 for pMLC (Cell Signaling Antibody #3671).

    Techniques: Immunofluorescence