pmlc (Cell Signaling Technology Inc)

Cell Signaling Technology Inc is a verified supplier

Cell Signaling Technology Inc manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Cell Signaling Technology Inc pmlc

( A,B ) Representative immunofluorescence images <t>of</t> <t>paxillin</t> and F-actin (phalloidin) ( A ), and <t>pMLC</t> and F-actin (phalloidin) ( B ) immunostains are shown in inverted black and white (ibw) contrast as well as color composites (DAPI signal also included). Scale bar = 10 µm. ( C ) Compared to WT cells (n=243), lamella size is significantly increased in DLC1 KD cells (n=290, p-adjusted<0.001) and DLC1 KO cells (n=242, p-adjusted<0.001), while DLC1 rescue cells (n=57) show significantly lower lamella sizes than both WT (p-adjusted=0.0098) and KO cells (p-adjusted<0.001). ( D ) DLC1 KD cells (n=290) display a significant increase in the total area of focal adhesions per cell (p-adjusted<0.001) compared to WT cells (n=243), while DLC1 KO cells (n=242) do not show a significant difference (p-adjusted=0.09). Rescue cells show a significant decrease in the total area of focal adhesions (p-adjusted<0.001). ANOVA plus Tukey’s honestly significant difference test.

Pmlc, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmlc/pmc13068432-322-16-17
Average 86 stars, based on 1 article reviews

pmlc - by Bioz Stars, 2026-11

86/100 stars

Images

1) Product Images from "GTPase-activating protein DLC1 spatio-temporally regulates Rho signaling"

Article Title: GTPase-activating protein DLC1 spatio-temporally regulates Rho signaling

Journal: eLife

doi: 10.7554/eLife.90305

( A,B ) Representative immunofluorescence images of paxillin and F-actin (phalloidin) ( A ), and pMLC and F-actin (phalloidin) ( B ) immunostains are shown in inverted black and white (ibw) contrast as well as color composites (DAPI signal also included). Scale bar = 10 µm. ( C ) Compared to WT cells (n=243), lamella size is significantly increased in DLC1 KD cells (n=290, p-adjusted<0.001) and DLC1 KO cells (n=242, p-adjusted<0.001), while DLC1 rescue cells (n=57) show significantly lower lamella sizes than both WT (p-adjusted=0.0098) and KO cells (p-adjusted<0.001). ( D ) DLC1 KD cells (n=290) display a significant increase in the total area of focal adhesions per cell (p-adjusted<0.001) compared to WT cells (n=243), while DLC1 KO cells (n=242) do not show a significant difference (p-adjusted=0.09). Rescue cells show a significant decrease in the total area of focal adhesions (p-adjusted<0.001). ANOVA plus Tukey’s honestly significant difference test.

Figure Legend Snippet: ( A,B ) Representative immunofluorescence images of paxillin and F-actin (phalloidin) ( A ), and pMLC and F-actin (phalloidin) ( B ) immunostains are shown in inverted black and white (ibw) contrast as well as color composites (DAPI signal also included). Scale bar = 10 µm. ( C ) Compared to WT cells (n=243), lamella size is significantly increased in DLC1 KD cells (n=290, p-adjusted<0.001) and DLC1 KO cells (n=242, p-adjusted<0.001), while DLC1 rescue cells (n=57) show significantly lower lamella sizes than both WT (p-adjusted=0.0098) and KO cells (p-adjusted<0.001). ( D ) DLC1 KD cells (n=290) display a significant increase in the total area of focal adhesions per cell (p-adjusted<0.001) compared to WT cells (n=243), while DLC1 KO cells (n=242) do not show a significant difference (p-adjusted=0.09). Rescue cells show a significant decrease in the total area of focal adhesions (p-adjusted<0.001). ANOVA plus Tukey’s honestly significant difference test.

Techniques Used: Immunofluorescence

other:

Article Title: A Mechanotransduction-Aware Strategy for Enhancing MSC Potency via 3D Culture and Localized Delivery
Article Snippet: Phalloidin-TRITC was purchased from Invitrogen (USA).

Staining:

Article Title: Inflammasomes and DNA Damage Orchestrate Divergent Pyroptotic Fates in Neutrophils
Article Snippet: .. Cells were permeabilized with 0.1% Triton X, blocked with 2% BSA, and stained overnight with primary antibodies as follows-8-OHdG (1:1000) (Obtained from Merck), IL-1β (1:100) (Invitrogen) or pMLC (1:100) (Myosin light chain; Cell Signaling Technology, Boston, MA) overnight at 4°C. .. Cells were then washed and stained with secondary antibody anti-goat Alexa fluor 488 or anti-rabbit Alexa fluor 488 for 2 h at RT (1:500, Invitrogen) and mounted in DAPI-containing media (Invitrogen) ( ).

Blocking Assay:

Article Title: Patterns of Mitochondrial ATP Predict Tissue Folding
Article Snippet: .. After blocking, samples were incubated in blocking buffer containing primary antibodies against Tom20 (1:100; D8T4N, rabbit mAb; Cell Signaling 42406S), E-cadherin (1:500; clone 36, mouse mAb; BD 610182), or pMLC (1:50; Ser19, mouse mAb; Cell Signaling 3675S) for 48 hours at 4°C. ..

Article Title: Rho kinase and RND3 regulate the direct effect of estradiol-17β on oviductal tonus.
Article Snippet: Briefly, the lysates from oviductal isthmic smooth reproduction@bioscientifica.com Manuscript submitted for review to Reproduction D ow nloaded from https://academ ic.oup.com /reproduction/advance-article/doi/10.1093/reprod/xaag004/8439658 by guest on 27 January 2026 8 128 muscle tissues (25 μg/lane) were prepared, separated using SDS-PAGE (7%–13%), and 129 transferred to PVDF membranes. .. After blocking, the membranes were incubated with antibodies 130 against RhoA (sc-418, Santa Cruz Biotechnology, Santa Cruz, USA, RRID:AB_628218), ROCK 131 I (sc-17794, Santa Cruz Biotechnology, RRID:AB_628223), ROCK II (sc-398519, Santa Cruz 132 Biotechnology), RND1 (A06347, Boster Biological Technology, Wuhan, China), RND2 (13844- 133 1-AP, Proteintech, Wuhan, China, RRID:AB_2181817), RND3 (66228-1-lg, Proteintech), 134 MYPT1 (2634, Cell Signaling Technology, Massachusetts, USA, RRID:AB_915965), pMYPT1 135 (Thr696, 5163, Cell Signaling Technology, RRID:AB_10691830), pMYPT1 (Thr853, 4563, Cell 136 Signaling Technology, RRID:AB_1031185), MLC (8505, Cell Signaling Technology, 137 RRID:AB_2728760), pMLC (Thr18/Ser19, 3674, Cell Signaling Technology, 138 RRID:AB_2147464) or GAPDH (NB300-221SS, Novus Biologicals, Briarwood Avenue, USA, 139 RRID:AB_790028) diluted with Can Get Signal Solution 1 (NKB-201, TOYOBO) overnight at 140 4°C or for 2 h at room temperature. .. After washing with TBS-T, HRP-linked sheep anti-mouse 141 IgG (NA931, Cytiva, RRID:AB_772210), HRP-linked donkey anti-rabbit IgG (NA934, Cytiva, 142 RRID: AB_772206), or HRP-linked goat anti-mouse IgM (ab97230, Abcam, Cambridge, UK, 143 RRID:AB_10688258) diluted with Can Get Signal Solution 2 (NKB-301, TOYOBO) were 144 incubated to the membranes for 1 h at room temperature.

Incubation:

Article Title: GTPase-activating protein DLC1 spatio-temporally regulates Rho signaling
Article Snippet: .. Cells were incubated overnight with the primary antibody 1:250 dilution for paxillin (Abcam ab32084), 1:50 for pMLC (Cell Signaling Antibody #3671). ..

Review